Instrumental Methods Of Analysis

Unit 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert's law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectorsPhoto tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component analysis



Open Unit

Unit 2

IR Spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications


Flame Photometry

Principle, interferences, instrumentation and applications


Atomic absorption spectroscopy

Principle, interferences, instrumentation and applications


Nepheloturbidometry

Principle, instrumentation and applications



Open Unit

Unit 3

Introduction to chromatography


Adsorption and partition column chromatography

Methodology, advantages, disadvantages and applications.


Thin layer chromatography

Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.


Paper chromatography

Introduction, methodology, development techniques, advantages, disadvantages and applications


Electrophoresis

Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications



Open Unit

Unit 4

Gas chromatography

Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications


High performance liquid chromatography (HPLC)

Introduction, theory, instrumentation, advantages and applications.



Open Unit

Unit 5

Ion exchange chromatography

Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications


Gel chromatography

Introduction, theory, instrumentation and applications


Affinity chromatography

Introduction, theory, instrumentation and applications



Open Unit